Fig. 6. Investigating the Effects of Stimulation Location.

The left panel (A) shows a serotonin double peak response in a single animal (experiment 9). The right side (B) shows a serotonin single peak response in a single animal (experiment 11). In each experiment, control files were collected with three different SE placements (i.-iii.) while the WE remained in the same place. The SE was removed from the brain and reinserted twice, anterior (i.) and posterior (iii.) to the original (ii.) placement.

Figure 6A depicts three double peak signals obtained in one animal. Each signal was collected by stimulating a different location along the MFB moving anterior (i) to posterior (iii). The second peak amplitude increases with the anterior placement of the SE and decreases posterior. In the third signal (iii), and most posterior SE placement, the second peak is almost completely eliminated. In Fig. 6B the experiment was repeated when a single peak was observed, and movement of the SE did not cause a double peak to appear. This allows us to conclude that while the stimulation along the MFB controls the amplitude of the second peak, the presence of a single or double peak remains wholly dependent on the CFM placement within the mPFC layers. We next study the discrete reuptake mechanisms tied to each domain.