Table 2.
Central composite design (CCD) with observed response of the dependent variables from MAE of avocado seeds.
| Independent variables | Phenolic compounds | Antioxidant activity | |||||
|---|---|---|---|---|---|---|---|
| Run order | X 1 (%) | X 2 (W) | X 3 (min) | TPC (mg·GAE/g) | TFC (mg·QE/g) | DPPH (%inhibition) | ABTS (%inhibition) |
| 1 | 40 | 80 | 1 | 52.99 | 0.98 | 22.93 | 17.59 |
| 2 | 80 | 80 | 1 | 47.25 | 0.66 | 24.86 | 11.21 |
| 3 | 40 | 400 | 1 | 74.64 | 8.89 | 44.31 | 39.6 |
| 4 | 80 | 400 | 1 | 64.76 | 5.90 | 39.88 | 35.63 |
| 5 | 40 | 80 | 5 | 65.94 | 5.59 | 38.23 | 37.62 |
| 6 | 80 | 80 | 5 | 66.34 | 9.14 | 32.52 | 35.47 |
| 7 | 40 | 400 | 5 | 76.29 | 19.70 | 79.76 | 60.66 |
| 8 | 80 | 400 | 5 | 77.83 | 16.65 | 73.95 | 56.18 |
| 9 | 40 | 240 | 3 | 77.06 | 10.92 | 49.64 | 46.40 |
| 10 | 80 | 240 | 3 | 78.71 | 9.70 | 47.56 | 40.66 |
| 11 | 60 | 80 | 3 | 68.73 | 6.43 | 32.5 | 50.77 |
| 12 | 60 | 400 | 3 | 89.39 | 15.70 | 62.11 | 80.32 |
| 13 | 60 | 240 | 1 | 72.79 | 10.14 | 39.8 | 44.81 |
| 14 | 60 | 240 | 5 | 79.16 | 21.45 | 68.66 | 73.18 |
| 15 | 60 | 240 | 3 | 83.52 | 15.48 | 54.35 | 68.92 |
| 16 | 60 | 240 | 3 | 80.45 | 15.23 | 52.59 | 67.44 |
| 17 | 60 | 240 | 3 | 84.66 | 16.10 | 57.68 | 70.33 |
X 1 = ethanol concentration; X2 = microwave power; X3 = extraction time; TPC = total phenolic content; TFC = total flavonoid content; DPPH = 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability; ABTS = 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) scavenging ability.