Figure 6.

The weight of harvested spleen was presented in (A) where representative images of harvested spleen presented healthy mice (fed with olive oil), untreated tumor mice (fed with olive oil), mice fed with 5 mg/kg eupatorin in olive oil, and mice fed with 20 mg/kg eupatorin in olive oil. (B-E) Representative images of the spleen tissue section harvested from (B) healthy mice, (C) untreated 4T1 tumor mice (fed with olive oil), (D) 4T1 tumor mice fed with 5 mg/kg body weight (BW) eupatorin in olive oil, and (E) breast cancer mice fed with 20 mg/kg BW eupatorin in olive oil. Excised tissue sections were stained with hematoxylin and eosin staining and viewed under the microscope (Nikon) at 400× magnification. Scale bar: 50 µm. (B) Normal distribution of white pulp (WP) and red pulp (RP) in the healthy spleen. (C) Diffuse WP and RP distribution with the presence of giant cells (black arrow) in the untreated mouse. Statistical analysis was performed using the unpaired t test. Data are presented as mean values ± SD of n = 3 independent experiments (*statistical significance [P < .05] between healthy mice and tumor mice).