Table 3.
Precision, accuracy, extraction efficiency, and matrix effect of rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine, hirsutine, and hirsuteine in mouse blood (n = 6).
| Compound | Concentration (ng/mL) | Accuracy (%) | Precision RSD (%) | Matrix Effect |
Recovery | ||
|---|---|---|---|---|---|---|---|
| Intraday | Interday | Intraday | Interday | ||||
| Rhynchophylline | 1 | 105.2 | 108.4 | 12.0 | 14.1 | 106.7 | 68.7 |
| 4 | 101.6 | 93.4 | 9.1 | 10.1 | 108.4 | 67.6 | |
| 90 | 97.1 | 106.6 | 10.6 | 7.4 | 103.6 | 64.6 | |
| 900 | 103.0 | 98.6 | 4.9 | 8.7 | 102.0 | 72.0 | |
| Isorhynchophylline | 1 | 108.8 | 109.0 | 14.0 | 11.1 | 94.1 | 71.0 |
| 4 | 97.6 | 90.6 | 11.6 | 6.8 | 103.3 | 83.5 | |
| 90 | 98.8 | 109.8 | 4.7 | 7.5 | 100.8 | 69.5 | |
| 900 | 102.9 | 96.3 | 5.1 | 2.5 | 95.6 | 79.5 | |
| Corynoxeine | 1 | 97.8 | 90.7 | 11.3 | 13.3 | 106.1 | 75.9 |
| 4 | 100.7 | 97.6 | 12.5 | 8.9 | 105.6 | 69.6 | |
| 90 | 99.0 | 104.6 | 5.1 | 12.2 | 107.4 | 73.9 | |
| 900 | 95.5 | 96.3 | 6.8 | 4.5 | 106.6 | 81.3 | |
| Isocorynoxeine | 1 | 86.5 | 110.4 | 9.5 | 12.1 | 108.4 | 79.2 |
| 4 | 97.0 | 92.9 | 11.4 | 11.7 | 105.9 | 86.8 | |
| 90 | 107.4 | 93.1 | 9.0 | 12.3 | 106.1 | 81.4 | |
| 900 | 101.3 | 100.2 | 7.2 | 6.4 | 104.0 | 80.9 | |
| Hirsutine | 1 | 107.6 | 92.7 | 11.9 | 14.1 | 103.9 | 78.8 |
| 4 | 97.1 | 97.0 | 6.1 | 13.5 | 102.4 | 72.6 | |
| 90 | 107.0 | 96.0 | 5.9 | 10.5 | 109.4 | 70.3 | |
| 900 | 104.3 | 97.8 | 5.0 | 9.0 | 107.8 | 76.4 | |
| Hirsuteine | 1 | 98.7 | 109.0 | 13.6 | 12.0 | 103.6 | 73.3 |
| 4 | 108.1 | 97.3 | 7.9 | 8.5 | 105.1 | 64.4 | |
| 90 | 102.1 | 94.7 | 8.6 | 10.2 | 105.6 | 76.1 | |
| 900 | 100.2 | 105.9 | 10.0 | 3.2 | 108.2 | 65.0 | |