FIG 7.
Effect of SGNHext length on substrate specificity of C-terminal OafA and OafB. Dot blot for acetylated abequose (αO:5, blue) on basal Salmonella enterica serovar Typhimurium LPS after incubation with purified C-terminal OafA and OafB and pNPA as an acetyl group donor; 10 μM OafA and 20 μM OafB were used in these reactions. αCore antibody (green) was used as a loading control. WT acetylated LPS was used as a positive control. +, Active protein; –, heat-treated protein. Representative of n = 3 repeats. “C-long” constructs comprise the SGNH domain with full SGNHext, “C-short” constructs comprise the SGNH domain with fewer SGNHext residues to expose the SGNH domain active site. See Fig. 1 for details of the C-terminal OafA and OafB constructs.