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. 2020 Jul 20;44(4):1365–1374. doi: 10.3892/or.2020.7697

Figure 1.

Figure 1.

Cyclin D1 is highly expressed in NSCLC cells and required for the malignant phenotype. (A) IHC staining analysis of cyclin D1 expression in 30 cases of matched NSCLC patient tissues and adjacent tissues. Left panel, representative IHC staining results of cyclin D1 (scale bar, 50 µm). Right panel, IHC score. (B) IB analysis of cyclin D1 expression in immortalized lung epithelial cells and NSCLC cells. (C) Cell viability of HCC827 (left) and H1975 (right) cells expressing sgCtrl or sgCyclin D1. Top, IB analysis of cyclin D1 expression. Bottom, cell viability assessed by an MTS assay. (D) Colony formation of HCC827 and H1975 cells expressing sgCtrl or sgCyclin D1 (scale bar, 200 µm). (E-G) In vivo tumor growth of HCC827 cells expressing sgCtrl or sgCyclin D1: (E) Tumor volume, (F) images of the tumors (scale bar, 1 cm), (G) tumor weight. ***P<0.001. NSCLC, non-small cell lung cancer; IHC, immunohistochemistry; IB, immunoblot; Ctrl, control; sgCyclin D1, single-guide RNA targeting cyclin D1.