Expression of LINC00958 is upregulated in CRC cell lines. (A) The expression of LINC00958 was examined in several CRC cell lines (GEO, SNU-C1, COLO205, HCT-116, HT-29 and SW480) using reverse transcription-quantitative PCR. The results were generated from three independent repeats (N=3). Data are expressed as mean ± standard deviation. Statistical comparisons were made by a two-tailed Student's t test. ***P<0.001 vs. NCM460. (B) The expression of LINC00958 was determined using western blotting after transfection with shRNA-LINC00958-1 or shRNA-LINC00958-2. All experiments were repeated three times independently (N=3). Data are expressed as mean ± standard deviation. Statistical comparisons were made by a two-tailed Student's t test. **P<0.01, ***P<0.001 vs. shRNA-NC. shRNA, short hairpin RNA; NC, negative control.