Figure 1.

RES inhibits viability and promotes apoptosis of AGS cells. (A) Cell viability was detected by CCK-8 assay after RES treatment (5–80 µM) for 24, 48 and 72 h. (B) Following exposure to RES (10, 20 and 40 µM) for 48 h, FITC-Annexin V/PI assays and flow cytometry were performed to detect cell apoptosis. (C) The percentage of apoptotic cells (Annexin V-FITC⁺/PI⁺ quadrant and Annexin V-FITC⁺/PI⁻ quadrant) was determined. (D and E) The protein expression levels of CHOP and PARP were detected by western blotting upon RES administration for 48 h. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001. RES, resveratrol; DDP, cisplatin; CCK-8, Cell Counting Kit-8; PARP, poly-ADP-ribose polymerase; CHOP, CCAAT/enhancer binding protein homologous protein.