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. 2020 Jul 31;44(4):1605–1615. doi: 10.3892/or.2020.7708

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — RES and DDP synergistically inhibit AGS cell viability. (A) Cell viability was determined by CCK-8 assay after cotreatment with different doses of RES and DDP for 48 h. (B) Following RES (20 µM) and DDP (1 µg/ml) combination treatment for 24, 48, 72 and 96 h, cell viability was detected by CCK-8 assay. (C) The morphological changes in AGS cells were determined after RES (20 µM) and DDP (1 µg/ml) cotreatment for 48 h (magnification, ×100). (D) Following RES (20 µM)/DDP (1 µg/ml) combination treatment for 48 h, colony-forming assays were performed. (E) Colonies in each well were counted. The results are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001. RES, resveratrol; DDP, cisplatin; CCK-8, Cell Counting Kit-8; CTRL, control; COM, combination treatment.