Figure 3.

miR-137 suppresses the increase in ROS caused by hypoxia to suppress apoptotic cell death. (A) DCFDA and MitoSOX red staining were performed to detect the production of total ROS and mitochondrial ROS in MCF-7 BCSCs in normoxia or in hypoxia following 1 µM MitoQ10 or 10 µM NAC treatment. (B) Expression of mitochondrial ROS in miR-137 mimics-treated BCSCs at 12 and 24 h time points. (C) Apoptotic cell death of miR-137-overexpressing BCSCs in normoxic or hypoxic condition by Annexin V/PI double staining at 0, 12, 24 h time points (left panel). Apoptotic cell death by statistical analysis (right panel). *P<0.05 vs. NC group. BCSCs, breast cancer stem-like cells; ROS, reactive oxygen species.