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. 2020 Aug 3;44(4):1627–1637. doi: 10.3892/or.2020.7714

Figure 4.

Figure 4.

miR-137 restores the cell mitochondrial dysfunction by inhibiting mitophagy. (A) Western blotting was performed to detect the protein levels of Fundc1, NIX, LC3, Tom20 and VADC in BCSCs after introduction of miR-137 mimics. (B) The mtDNA levels in BCSCs after introduction of miR-137 mimics were assessed by RT-qPCR. *P<0.05, vs. Normoxia group; #P<0.05, vs. NC mimics group. (C) Citrate synthase activity was measured by Citrate Synthase Activity Colorimetric Assay Kit. *P<0.05, vs. Normoxia group; #P<0.05, vs. NC mimics group. (D) Synthesized ATP was analyzed by ATP synthase Assay Kit. *P<0.05, vs. Normoxia group; #P<0.05, vs. NC mimics group. (E) The mtDNA transcription activity was measured by assessing the expression of COXI and ND1. *P<0.05, vs. Normoxia group; #P<0.05, vs. NC mimics group. BCSCs, breast cancer stem-like cells; mtDNA, mitochondrial DNA; NIX, also known as beclin-2 (BCL2); Fundc1, Fun14 domain-containing protein 1; VADC, voltage-dependent anion channel; LC3, light chain 3; Tom20, mitochondrial import receptor subunit TOM20 homolog; COXI, cytochrome c oxidase I; ND1, NADH-ubiquinone oxidoreductase chain 1; NC, negative control.