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. 2020 Aug 3;44(4):1627–1637. doi: 10.3892/or.2020.7714

Figure 5.

Figure 5.

miR-137 regulates mitophagy via targeting Fundc1. (A) Western blotting was performed to detect Fundc1, Tom20, and Tim23 after introduction of miR-137 mimics or treatment with LW-6 after 12 or 24 h (upper panel). Fundc1, Tom20, and Tim23 protein expression by statistical analysis (lower panels). *P<0.05, vs. 12 h NC mimics group; #P<0.05, vs. 24 h NC mimics group; †P<0.05, vs. 12 h Mock group; ▲P<0.05, vs. 24 h Mock group. (B) The protein expression level of LC3 (upper panel). LC3-II protein expression by statistical analysis (lower panel). *P<0.05, vs. 12 h NC mimics group; #P<0.05, vs. 24 h NC mimics group; †P<0.05, vs. 12 h Mock group; ▲P<0.05, vs. 24 h Mock group. (C) The colocalization of LC3 with mitochondria (left panel). LC3 dots colocalized with mitochondria per cell by statistical analysis (right panel). *P<0.05, vs. NC mimics group. Fundc1, Fun14 domain-containing protein 1; LC3, light chain 3; Tom20, mitochondrial import receptor subunit TOM20 homolog; Tim23, mitochondrial import inner membrane translocase subunit Tim23; NC, negative control.