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. 2020 Jul 20;44(4):1511–1523. doi: 10.3892/or.2020.7699

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — lncRNA promotes the migration of BC cells by downregulating miR-340-5p. (A and B) Transfection of miR-340-5p mimics significantly upregulated the expression of miR-340-5p in MDA-MB-231 and MCF7 cells compared with the miR-ctrl group, ***P<0.001, compared with the miR-ctrl group. Transfection of miR-340-5p inhibitor obviously downregulated the expression of miR-340-5p compared with the inhibitor NC group, indicating successful transfection. ***P<0.001, compared with the inhibitor NC group. (C and D) Overexpression of miR-340-5p had no significant effect on BC cell viability, as confirmed by CCK-8 assay results. (E-H) Overexpression of miR-340-5p mimics decreased the speed of the wound closure by ~66% in MDA-MB-231 cells and by ~72% in MCF7 cells (both **P<0.01, compared with the miR-ctrl group). (I-K) Transwell invasion assays showed that overexpression of miR-340-5p mimics could inhibit cell invasion in MDA-MB-231 and MCF7 cells compared with the negative control group. **P<0.01, compared with the miR-ctrl group. (L) Overexpression or inhibition of miR-340-5p had no influence on the expression of lncPCNAP1 in MCF7 cells. Results are representative of three independent experiments. Data are shown as mean ± SD. BC, breast cancer; lncRNA, long noncoding RNA; PCNAP1, proliferating cell nuclear antigen pseudogene 1.