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. 2020 Jul 20;44(4):1511–1523. doi: 10.3892/or.2020.7699

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — lncRNA PCNAP1 promotes BC metastasis by downregulating miR-340-5p, then upregulating SOX4. (A) Immunohistochemistry results showed that SOX4 was significantly increased in BC tissues compared with that in adjacent normal tissues (magnification, ×100). (B) RT-qPCR validation of the immunohistochemistry results. **P<0.01, compared with adjacent tissues. (C) SOX4 expression was increased in metastatic BC tissues compared with non-metastatic tissues. ***P<0.001, compared with non-metastatic tissues. (D) There was a positive correlation between the expression levels of lncRNA PCNAP1 and SOX4 in metastatic tissues. (E-G) Knockdown of PCNAP1 (siPCNAP1#a and siPCNAP1#b) significantly suppressed the protein expression of SOX4 in MDA-MB-231 and MCF7 cells compared with the negative control group (siCtrl). ***P<0.001, compared with the siCtrl group. (H) There was a negative correlation between miR-340-5p and SOX4 expression in metastatic tissues. (I-K) miR-340-5p mimics decreased the protein expression of SOX4 compared with the miR-Ctrl group in MDA-MB-231 and MCF7 cells. ***P<0.001, compared with the miR-Ctrl group. In contrast, the miR-340-5p inhibitor increased the expression compared with the inhibitor NC group. ***P<0.001, compared with the inhibitor NC group. (L and M) Expression of SOX4 was upregulated by the overexpression plasmid. ***P<0.001, compared with the vector group. (N and O) Knockdown of lncRNA PCNAP1 decreased invasion which was restored by overexpression of SOX4. ***P<0.001, compared with the siCtrl group. Results are representative of three independent experiments. Data are shown as mean ± SD. BC, breast cancer; lncRNA, long noncoding RNA; PCNAP1, proliferating cell nuclear antigen pseudogene 1; SOX4, SRY-box transcription factor 4.