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. 2020 Aug 25;20:404. doi: 10.1186/s12935-020-01496-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The relationship between circ_0000517 and miR-326. a The targets of circ_0000517 were analyzed via circBank, StarBase and circinteractome. b, c miR-1296 and miR-326 levels were detected in HCCLM3, Huh7 and THLE-2 cells with transfection of si-circ_0000517#3 or si-NC. d The binding site of circ_0000517 and miR-326. e, f Luciferase activity was examined in HCCLM3 and Huh7 cells transfected with circ_0000517-WT or circ_0000517-MUT and miR-326 mimic or miR-NC. g miR-326 expression was measured in tumor and adjacent nontumor tissues from patients with hepatocellular carcinoma. h miR-326 level was examined in HCCLM3, Huh7 and THLE-2 cells. i The relationship between miR-326 and circ_0000517 levels in hepatocellular carcinoma tissues. ^*P < 0.05