Figure 4.

miR-326 is a target of SNHG3 in breast cancer. (A) The putative binding sites for miR-326 in the 3′-UTR (untranslated region) of SNHG3 (WT-SNHG3) are predicted by Starbase 2.0. The target sequences of the SNHG3-3′UTR were mutated (MT-SNHG3). (B) Luciferase activity was examined in MCF-7 cells co-transfected with miR-326 mimics or miR-NC, and luciferase reporter vector containing WT-SNHG3 or MT-SNHG3. WT, wild-type; MT, mutant-type. (C) Expression of SNHG3 in MCF-7 cells transfected with miR-NC or miR-326 mimics was determined by RT-qPCR. (D) Expression of miR-326 in MCF-7 cells transfected with sh-NC or sh-SNHG3 was determined by RT-qPCR. (E) Pearson's correlation analysis between miR-326 and SNHG3 expression in 48 breast cancer tissues. **P<0.01. SHNG3, small nucleolar RNA host gene 3; sh-SNHG3, SNHG3-knockdown group; sh-NC, negative control group.