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Gene primers for RT-PCR
| Gene | Sequence |
|---|---|
| Angiotensinogen (Agt) | Sense: AGCACGGACAGCACCCTATT |
| Antisense: AGAACTCATGGAGCCCAGTCA | |
| Angiotensin-converting enzyme (ACE) | Sense: CTGCCTCCCAACGAGTTAGAA |
| Antisense: CGGGACGTGGCCATTATATT | |
| Angiotensin II type I receptor (AT1R) | Sense: TATCACAGTGTGCGCGTTTCA |
| Antisense: TGGTAAGGCCCAGCCCTAT | |
| Renin | Sense: GCTACATGGAGAATGGGACTGAA |
| Antisense: ACCACATCTTGGCTGAGGAAAC | |
| Reference gene: elongation factor-1 (Ef-1) | Sense: GCAAGCCCATGTGTGTTGAA |
| Antisense: TGATGACACCCACAGCAACTG |
