Table II.
qPCR primers used for mRNA expression analysis.
| Target gene | Primer (5′-3′) |
|---|---|
| U6 | F-TCCGATCGTGAAGCGTTC |
| R-GTGCAGGGTCCGAGGT | |
| miR-143-3p | F-GTGAGATGAAGCACTGTAGC |
| R-GTGCAGGGTCCGAGGT | |
| MCM3AP-AS1 | F-GCTGCTAATGGCAACACTGA |
| R-AGGTGCTGTCTGGTGGAGAT | |
| TAK1 | F-TCTGGATGTCCCTGAGATCGT |
| R-GCTCACCTGACCAGGTTCTG | |
| GAPDH | F-AAGGTGAAGGTCGGAGTCAA |
| R-AATGAAGGGGTCATTGATGG |
F, forward; R, reverse; TAK1, transforming growth factor-β-activated kinase 1; MCM3AP-AS1, MCM3AP antisense 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; mRNA, messenger RNA; PCR, polymerase chain reaction.