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Two-dimensional 1H-13C ILV methyl HMQC spectra of GTPγS bound and ND-anchored protonated-myrArf1: ND. The peaks at approximately 13.5–14.1 ppm (marked with ) arise from natural abundance 13C signals of the terminal methyl moiety of the lipid acyl chains. The Leu and Val resonance assignments are stereospecifically ambiguous and are marked with *.
