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(A) Overlay of the 2D 1H-13C methyl ILV HMQC spectra of GTPγS bound forms of deuterated L8K-Arf1 (blue) and ND-anchored protonated-myrArf1-HIS:ND (red). The peaks marked by * belong to a small residual of the GDP bound form of L8K-Arf1. (B) Overlay of I74 Ile region of the 2D 1H-13C HMQC spectra for ND-anchored myrArf1 with either GTPγS bound (red), GMPPCP bound (green) or GDP bound (blue).
