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. 2020 Aug 12;20(4):112. doi: 10.3892/ol.2020.11973

Figure 2.

Figure 2.

Evaluation of autophagy effects on H2-induced lung cancer cell apoptosis. (A) The protein expression levels of p62, Beclin1, LC3BII and LC3BI, following treatment of A549 and H1975 cells with RAPA were detected using western blot analysis. (B) Cell apoptosis was investigated using a flow cytometry assay. Western blot analysis of the expression of cleaved-caspase 3 and cleaved-PARP in (C) A549 and (D) H1975 cells treated with H2 or H2+RAPA. (E) The protein expression levels of p62, Beclin1, LC3BII and LC3BI were detected using western blot analysis following treatment of A549 and H1975 cells with 3-MA. (F) Cell apoptosis was investigated using flow cytometry. Western blot analysis of the protein expression levels of cleaved-caspase 3 and cleaved-PARP following treatment of (G) A549 and (H) H1975 cells with H2 or H2+3-MA. *P<0.05. **P<0.01. LC3, light chain 3; RAPA, rapamycin; PARP, poly ADP-ribose polymerase; PI, propidium iodide; 3-MA, 3-methyladenine; H2, hydrogen gas.