Figure 3.

Knockdown of Beclin1 enhances the role of H₂ in promoting lung cancer cell apoptosis. (A) The knockdown efficiency of si-Beclin1 in A549 and H1975 cells was detected using western blot analysis. (B) Western blot analysis was used to detect the protein expression levels of p62, LC3BII and LC3BI in A549 and H1975 cells and the results were subsequently (C) quantified. (D) Flow cytometry assay was used to investigate cell apoptosis. The protein expression levels of cleaved-caspase 3 and cleaved-PARP were determined using western blot analysis in (E) A549 and (F) H1975 cells. *P<0.05 and **P<0.01. si, short interfering; LC3, light chain 3; PARP, poly ADP-ribose polymerase; PI, propidium iodide; H₂, hydrogen gas.