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. 2020 Aug 13;20(4):121. doi: 10.3892/ol.2020.11983

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — EVO induces apoptosis in A-375 cells. A-375 cells were treated with 10 µM EVO for 12 or 24 h. (A) Annexin-V/PI staining assay. The apoptotic rates were examined by flow cytometry. (B) The protein levels of caspase-3, caspase-9, BCL2, BAX and PARP1 were examined by western blotting. (C) The BCL2/BAX protein ratios were calculated. (D) JC-1 assay. The values of Δψm were examined by flow cytometry and expressed as the ratio of red to green fluorescence intensity. *P<0.05, **P<0.01 and ***P<0.001 vs. DMSO. EVO, evodiamine; PI, propidium iodide; PARP1, poly (ADP-ribose) polymerase 1; CF, cleaved form; Δψm, mitochondrial membrane potential.