TABLE 1.
Specimens used for evaluation of multiplex PCR
| Organism (no.) | Laboratory (no. of samples; location) | Specimen type (no.) | Reference assay (no. positive) | Reference(s) |
|---|---|---|---|---|
| SFGR (20) | National Institute of Health, Ricardo Jorge (18 samples; Portugal); CDC (2 samples; USA) | Blood/buffy coat (18), DNA (2) | Conventional PCR (18), real-time PCR (2), serology (18), culture (18) | 41, 42 |
| TGR (30) | Mahidol Oxford Tropical Medicine Research Unit (20 samples; Thailand); Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (10 samples; Lao PDR) | Blood/buffy coat (10), DNA (20) | Real-time PCR (30), serology (10), culture (10) | 35 |
| O. tsutsugamushi (30) | Mahidol Oxford Tropical Medicine Research Unit (20 samples; Thailand); Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (10 samples; Lao PDR) | Blood/buffy coat (10), DNA (20) | Real-time PCR (30), serology (10), culture (10) | 35 |
| E. chaffeensis (45) | Johns Hopkins University School of Medicine (USA); Mayo Clinic (USA); University of Texas Medical Branch (USA) | Blood (6), DNA (39) | Conventional PCR (12), real-time PCR (32), serology (10), morulae (1), culture (4) | 26, 43–45 |
| A. phagocytophilum (15) | Johns Hopkins University School of Medicine (USA) | Blood | Real-time PCR (15), serology (11), morulae (14), culture (4) | 26 |
| Bloodstream infections (17) | Johns Hopkins Hospital/University School of Medicine (USA) | Blood | Blood culture (17) | 26 |
| P. falciparum (28) | Johns Hopkins Hospital/University School of Medicine (USA) | Blood | Blood smear (28), real-time PCR (22) | 36 |
| P. vivax (5) | Johns Hopkins Hospital/University School of Medicine (USA) | Blood | Blood smear (5), real-time PCR (5) | 36 |
| E. chaffeensis (6) | USA reference laboratory (Mayo Clinic) | Blood | Real-time PCR (6) | 45 |
| Convalescent from E. chaffeensis or A. phagocytophilum (3) | Johns Hopkins University School of Medicine (USA) | Blood | Conventional PCR (3), serology (3), morulae (2), culture (3) | 26 |