TABLE 2.

Primers and probes used for multiplex PCR

Target pathogen	Accession no.	Target gene	Forward primer	Reverse primer	Probe
SFGR	NC_009882.1	SFGR ompA consensus	TTGTCAGGCTCTGAAGCTAAAC	AGCACCTGCCGTTGTGATATC	FAM-TAGCCGCAGTCCCTACAACACCGC-BHQ1
TGR	M28481	R. typhi 17-kDa antigen gene TGR optimized	ACTTGGTTCTCAATTCGGTCAC	CAGACTTGCACCGATTTGTCC	TXR-TGCCCCAAGTAATGCGCCTACACC-BHQ1
O. tsutsugamushi	AY836148.1	Orientia tsutsugamushi strain Kato 56-kDa antigen gene	GGTGGTAATGCTTTCGCTAATCAG	TGCTGCTTCTTGCGCCTGTAG	CY3/HEX-TGCTGCTGTTGCTGCCCTTGCC-BHQ1
E. chaffeensis	AF181986	vlpt	CTAATTCTGATTTACACGAGTCTTC	GCATCATCTTCGAATTGAACTTC	TET-TTGAGTTACCTGGTCC-BHQ1
A. phagocytophilum	Many	msp2 (5’ conserved domain)	GAAGATGAWGCTGATACAGTA	CAACHGCCTTAGCAAACT	Cy5-TTATCAGTCTGTCCAGTAACA-BHQ1