TABLE 2.
MICs of CZA for the strains tested and detection limits of the SuperCAZ/AVI screening medium
| Category and strain | Species | Origin | MIC of CZAa (mg/liter) | CZA susceptibility or resistanceb | Resistance determinant(s) | Lower limit of detection (CFU/ml)c
in: |
|
|---|---|---|---|---|---|---|---|
| Culture | Stoolsc | ||||||
| Enterobacterales | |||||||
| R1433 | Enterobacter cloacae | France | 0.19 | S | Wild type | >108 | >107 |
| R254 | Klebsiella pneumoniae | France | 0.064 | S | Porin deficiency, SHV, AmpC | 106 | 106 |
| R1233 | Escherichia coli | France | 0.5 | S | ACC-1 | >108 | >107 |
| R1241 | Klebsiella pneumoniae | USA | 1.5 | S | ACT-1 | 106 | 106 |
| R2077 | Escherichia coli | Switzerland | 0.5 | S | ACC-1 | 107 | 107 |
| R1291 | Escherichia coli | USA | 0.032 | S | OXA-1 | >108 | >107 |
| R1335 | Escherichia coli | France | 0.064 | S | TEM-1 | 108 | >107 |
| R941 | Enterobacter cloacae | Switzerland | 1.5 | S | TEM-1 | 107 | 107 |
| R1906 | Escherichia coli | France | 0.75 | S | SHV-12 | >108 | >107 |
| R2180 | Enterobacter cloacae | France | 2 | S | GES-5 | >108 | >107 |
| N23 | Escherichia coli | Switzerland | 0.032 | S | CTX-M-15 | 108 | >107 |
| N41 | Escherichia coli | Switzerland | 0.064 | S | CTX-M-9 | 108 | >107 |
| N44 | Escherichia coli | France | 0.125 | S | CTX-M-15 | >108 | >107 |
| N71 | Escherichia coli | Switzerland | 0.032 | S | CTX-M-15 | >108 | >107 |
| R1039 | Escherichia coli | Vietnam | 0.25 | S | VEB-1, OXA-10, TEM-1 | >108 | >107 |
| R1104 | Klebsiella pneumoniae | Thailand | 0.75 | S | VEB-1 | >108 | >107 |
| R1103 | Klebsiella pneumoniae | Thailand | 0.5 | S | VEB-1 | >107 | >107 |
| R144 | Escherichia coli | France | 0.75 | S | VEB-1 | >108 | >107 |
| R1105 | Klebsiella pneumoniae | Thailand | 0.25 | S | VEB-1 | >108 | >108 |
| R2658 | Escherichia coli | France | 0.125 | S | VEB-1, TEM-1, OXA-10 | >108 | >107 |
| R3659 | Escherichia coli | USA | 0.5 | S | KPC-2 (E. coli DH10B/pBR322 blaKPC-2) | >108 | >108 |
| R99 | Klebsiella pneumoniae | France | 0.5 | S | KPC-2 | 107 | 105 |
| R3521 | Klebsiella pneumoniae | Switzerland | 1.5 | S | KPC-2 | 105 | 106 |
| R3668 | Escherichia coli | USA | 0.064 | S | KPC-2 [E. coli DH10B/pBC SK(+) blaKPC-2] | >108 | >108 |
| R82 | Escherichia coli | France | 0.047 | S | KPC-2 | >108 | >107 |
| R91 | Klebsiella pneumoniae | France | 0.75 | S | KPC-2 | >108 | >107 |
| R94 | Klebsiella pneumoniae | France | 2 | S | KPC-2 | >108 | >107 |
| R3485 | Klebsiella pneumoniae | Switzerland | 1 | S | KPC-2 | 106 | 106 |
| R3486 | Klebsiella pneumoniae | Switzerland | 1 | S | KPC-2 | 108 | >107 |
| R3488 | Klebsiella pneumoniae | Switzerland | 1 | S | KPC-2 | 107 | >107 |
| R3522 | Klebsiella pneumoniae | Switzerland | 1.5 | S | KPC-2 | >108 | >107 |
| R132 | Klebsiella pneumoniae | France | 1 | S | KPC-2 | >108 | >107 |
| R297 | Klebsiella pneumoniae | France | 0.25 | S | KPC-2, OXA-1 | >108 | >107 |
| R100 | Klebsiella pneumoniae | France | 1.5 | S | KPC-11 | 108 | >107 |
| R22 | Escherichia coli | France | 0.094 | S | OXA-48 | >108 | >107 |
| R740 | Escherichia coli | The Netherlands | 1 | S | OXA-48 | >108 | >107 |
| R19 | Klebsiella pneumoniae | France | 0.5 | S | OXA-48 | 107 | 106 |
| R23 | Klebsiella pneumoniae | France | 0.5 | S | OXA-48 | 107 | 107 |
| N59 | Escherichia coli | Switzerland | 0.023 | S | OXA-181 | >108 | >107 |
| R131 | Klebsiella pneumoniae | France | 1.5 | S | OXA-181 | 108 | >107 |
| R3338 | Klebsiella pneumoniae | USA | 24 | R | CMY-4, VIM-1 | 102 | 101 |
| R169 | Klebsiella pneumoniae | USA | 24 | R | VIM-19 | 101 | 101 |
| N284 | Enterobacter cloacae | Switzerland | 48 | R | VIM-1 | 101 | 101 |
| R48 | Klebsiella pneumoniae | France | >256 | R | VIM-1 | 101 | 101 |
| R61 | Escherichia coli | France | 24 | R | VIM-1, SHV-12 | 102 | 101 |
| R63 | Klebsiella pneumoniae | France | 24 | R | VIM-19 | 101 | 101 |
| N6 | Escherichia coli | Switzerland | >256 | R | NDM-5 | 102 | 102 |
| R464 | Escherichia coli | France | >256 | R | NDM-4, OXA-1 | 101 | 101 |
| R466 | Escherichia coli | France | >256 | R | NDM-4, OXA-1, CTX-M-15 | 102 | 101 |
| R3778 | Klebsiella pneumoniae | Spain | 48 | R | KPC-3 D179Y | 101 | 101 |
| R3780 | Klebsiella pneumoniae | Spain | >256 | R | KPC-3 G168N E169H | 101 | 101 |
| R3781 | Klebsiella pneumoniae | Spain | 64 | R | KPC-3 E169P L172T | 101 | 101 |
| R3776 | Klebsiella pneumoniae | Spain | 96 | R | KPC-3 D179Y | 101 | 101 |
| R3777 | Klebsiella pneumoniae | Spain | >256 | R | KPC-3 D179Y A172T | 101 | 101 |
| N435 | Klebsiella pneumoniae | Switzerland | >256 | R | KPC-41 | 101 | 101 |
| N859 | Klebsiella pneumoniae | Switzerland | >256 | R | KPC-50 | 101 | 101 |
| R3671 | Escherichia coli | USA | >128 | R | KPC-2 (E. coli DH10B/pBR322 KPC-2 D179M) | 102 | 102 |
| R3779 | Klebsiella pneumoniae | Spain | 128 | R | KPC-3 D179Y | 102 | 102 |
| R72 | Escherichia coli | France | 128 | R | IMP-1 | 101 | 101 |
| R73 | Klebsiella pneumoniae | France | >256 | R | IMP-1 | 101 | 101 |
| Pseudomonas aeruginosa | |||||||
| R1553 | Pseudomonas aeruginosa | France | 1.5 | S | None (wild type) | >108 | 107 |
| R2267 | Pseudomonas aeruginosa | France | 0.75 | S | None (wild type) | >108 | 107 |
| N382 | Pseudomonas aeruginosa | Switzerland | 0.38 | S | None (wild type) | 106 | 107 |
| N339 | Pseudomonas aeruginosa | Switzerland | 0.5 | S | None (wild type) | >108 | 107 |
| N146 | Pseudomonas aeruginosa | Switzerland | 4 | S | GES-5 | 104 | 106 |
| N254 | Pseudomonas aeruginosa | Switzerland | 1 | S | None (wild type) | >108 | 108 |
| N214 | Pseudomonas aeruginosa | Switzerland | 0.5 | S | None (wild type) | >108 | 107 |
| R1187 | Pseudomonas aeruginosa | Belgium | 4 | S | BEL-2 | 106 | 105 |
| R1188 | Pseudomonas aeruginosa | Brazil | 2 | S | CTX-M-2 | >108 | >107 |
| R3451 | Pseudomonas aeruginosa | France | 1 | S | GES-6 | 106 | 107 |
| R3680 | Pseudomonas aeruginosa | USA | 24 | R | Unknown mechanism | 101 | 102 |
| R3681 | Pseudomonas aeruginosa | USA | 32 | R | Unknown mechanism | 101 | 101 |
| R3682 | Pseudomonas aeruginosa | USA | 64 | R | Unknown mechanism | 101 | 101 |
| R3683 | Pseudomonas aeruginosa | USA | >256 | R | Unknown mechanism | 101 | 101 |
| R1308 | Pseudomonas aeruginosa | France | >256 | R | OXA-28 | 101 | 102 |
| R1311 | Pseudomonas aeruginosa | France | 12 | R | OXA-32 | 101 | 101 |
| R609 | Pseudomonas aeruginosa | Turkey | 64 | R | VIM-2 | 101 | 101 |
| R50 | Pseudomonas aeruginosa | France | 24 | R | VIM-2 | 102 | 101 |
| R51 | Pseudomonas aeruginosa | France | >256 | R | VIM-2 | 101 | 101 |
| R52 | Pseudomonas aeruginosa | France | 16 | R | VIM-2 | 101 | 101 |
| R54 | Pseudomonas aeruginosa | France | >256 | R | VIM-2 | 102 | 101 |
| R598 | Pseudomonas aeruginosa | France | 24 | R | VIM-2 | 101 | 101 |
| R599 | Pseudomonas aeruginosa | France | 16 | R | VIM-2 | 101 | 101 |
| R600 | Pseudomonas aeruginosa | Japan | 16 | R | VIM-2 | 102 | 101 |
| R604 | Pseudomonas aeruginosa | The Netherlands | 12 | R | VIM-2 | 101 | 101 |
| R608 | Pseudomonas aeruginosa | France | 16 | R | VIM-2 | 102 | 102 |
| R610 | Pseudomonas aeruginosa | France | 32 | R | VIM-2 | 102 | 102 |
| N885 | Pseudomonas aeruginosa | Switzerland | >256 | R | NDM-1 | 101 | 101 |
| N520 | Pseudomonas aeruginosa | Switzerland | >256 | R | NDM-1 | 101 | 101 |
| N521 | Pseudomonas aeruginosa | Switzerland | >256 | R | NDM-1 | 101 | 101 |
| R186 | Pseudomonas aeruginosa | France | 16 | R | NDM-6 | 102 | 102 |
| R2760 | Pseudomonas aeruginosa | France | >256 | R | NDM-1 | 102 | 101 |
a
CZA, ceftazidime-avibactam. MICs of CZA were determined using Etest.
b
R, resistant; S, susceptible.
c
Underlined CFU counts are considered negative results (cutoff values were set at >103 CFU/ml).