Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The formulation of primers for MinION sequencing and the entry of the various PCR products into the sequencing library preparation protocol. In addition to the basic primers, we have tested the addition of barcode adaptor (BCA) primers or barcodes and flanking regions (BC# primers, with # being the barcode number with reference to nanopore PCR ligation barcode sequences).
