FIG 4.

Carbapenemase-encoding plasmid diversity among and between CNSC genomes. CNSC genome contigs were compared to each other and to sequences deposited in the National Center for Biotechnology Information (NCBI). Sequences were aligned to one another with EasyFig. Sequence names are in the format “isolate_contig” based on hybrid assembly or correspond to the sequence name from NCBI. Bacterial species and sequence type are listed, where available. Open reading frames are colored by function (blue, mobilization; pink, carbapenemase; red, other antibiotic resistance; gray, metal interacting; orange, other/hypothetical). Antibiotic resistance genes, metal-interacting operons, and type IV secretion system components are labeled. Gray blocks between sequences indicate regions of >5 kb with >98% nucleotide identity, with darker shading indicating higher identity. Nucleotide identity between the bla_KPC-3-encoding Tn4401-like regions of YDC693_4 and YDC697-2_6 (from two isolates of different Citrobacter species from the same patient) is noted with white text in panel C.