TABLE 1.
Analytical limits of detection for SARS-CoV-2
| Assay | No. of SARS-CoV-2 detected/no. tested at a viral concn (cp/ml) of: |
Exp. LODa | Probit LOD | |||||
|---|---|---|---|---|---|---|---|---|
| 3,160 | 1,000 | 316 | 100 | 32 | 10 | |||
| Abbott m2000 | ND | ND | 20/20 | 19/20 | 20/20 | 16/20 | 32 | 53 |
| Roche Cobas | ND | ND | 20/20 | 20/20 | 20/20 | 20/20 | ≤10 | ≤10 |
| Panther Fusion | 5/5 | 5/5 | 5/5 | 20/20 | 15/20 | 1/20 | 100 | 74 |
| DiaSorin Simplexa | 5/5 | 5/5 | 20/20 | 19/20 | 14/20 | ND | 100 | 167 |
| GenMark ePlex | 5/5 | 5/5 | 5/5 | 19/20 | 8/20 | ND | 100 | 190 |
| Abbott ID NOW | 5/5 | 5/5 | 24/25 | 14/25 | 9/20 | 0/5 | 316 | 511 |
| CDC ABI 7500 (MP24) | 5/5 | 5/5 | 20/20 | 15/20 | 1/5 | ND | 316 | 237 |
| CDC LC 480 (MP24) | 5/5 | 5/5 | 19/20 | 14/20 | 1/5 | ND | 316 | 499 |
| CDC ABI 7500 (EZ1) | 5/5 | 5/5 | 20/20 | 20/20 | 13/20 | ND | 100 | 85 |
| CDC LC 480 (EZ1) | 5/5 | 5/5 | 20/20 | 19/20 | 10/20 | ND | 100 | 185 |
a
Exp. LOD, experimental limit of detection; Probit LOD, Probit limit of detection; ND, not done; ABI 7500, Applied Biosystems 7500 Fast real-time PCR system; LC 480, Roche LightCycler 480 II real-time PCR system; MP24, Roche MagaPure 24 extractor; EZ1, Qiagen EZ1 extractor.