Table 1.
Fynomer cross-linker attachment sites tested
| Mutant | Cross-link point | Res. apart | Half-life (IAC) [min] | Half-life (PIP) [sec] | Res. flanking 1st Cys | Res. flanking 2nd Cys |
|---|---|---|---|---|---|---|
| D9CT44C | D9C + T44C | 34 | 42.2 | — | YCY | TCG |
| D9CE46C | D9C + E46C | 36 | 10.4 | 2.1 | YCY | GCT |
| N11CH21C | N11C + H21C | 9 | — | — | YCA | FCK |
| N11CT44C | N11C + T44C | 33 | 11.8 | 3.5 | YCA | TCG |
| H21CK25C | H21C + K25C | 3 | — | — | FCK | ECF |
| H21CT43C | H21C + T43C | 21 | — | 3.0 | FCK | LCT |
| H21CT44C | H21C + T44C | 22 | 14.6 | 4.4[a] | FCK | TCG |
| H21CE46C | H21C + E46C | 24 | 16.4 | 8.6 | FCK | GCT |
| K25CT43C | K25C + T43C | 21 | — | 6.8 | ECF | LCT |
| L3CL29C[b] | L3C + L29C | 25 | — | 130 | TCF | ICE |
[a]
Includes uncross-linked protein.
[b]
Previously characterized with BSBCA.[6a]