Hydrodynamic diameter and polydispersity values for silica NP without GF peptide (top) and with GF peptide (bottom, shaded) across various aqueous solutions. All samples made in 5 mM sodium phosphate buffer, pH 7.4 with additional solutes as listed. Samples were incubated for 2 h to allow time to equilibrate, matching the equilibration period for the corresponding fluorescence anisotropy experiments. Average values are reported from 2 measurement replicates of 2 assay replicates (n = 4 total). Results were analyzed with a 1-way ANOVA and Tukey Test. Groupings of datasets with significant p-values marked with (*).

	Matrix	Hydrodynamic diameter, nm		Polydispersity
		Ave	% RSD	Ave	% RSD
Silica NP	Buffer	72.8	0.85	0.073	10
	0.29 mM NaCl	73.3	0.46	0.068	7.6
	29 mM NaCl	73.4	1.4	0.062	21
	1 M glucose	74.5	0.60	0.052	17
	0.29 mM NaCl + HS	79.1*	1.3	0.079	3.0
	29 mM NaCl + HS	101.7**	2.6	0.17	31
	1 M glucose + HS	82.0***	1.1	0.084	5.9
Silica NP with GF peptide	Buffer	73.7	0.62	0.049	9.6
	0.29 mM NaCl	73.8	0.71	0.053	13
	29 mM NaCl	73.5	0.60	0.032	12
	1 M glucose	73.8	0.69	0.070	4.7
	0.29 mM NaCl + HS	77.9*	2.0	0.080	12
	29 mM NaCl + HS	89.8***	0.51	0.11	16
	1 M glucose + HS	82.1***	1.4	0.098	8.3