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Effects of E2F3 depletion on bladder cancer SP phenotype. (a) E2F3 transcript levels by qRT-PCR in SP vs. NSP in J82 (left, p < 0.01) and T24 cells (right, p < 0.01). (b) Lentiviral shRNA-mediated E2F3 depletion in J82 cells (left, p < 0.01) results in decreased SP (center and right, p < 0.01). (c) Lentiviral shRNA-mediated E2F3 depletion in T24 cells (left, p < 0.01) results in decreased SP (center and right, p < 0.01). [Color figure can be viewed at wileyonlinelibrary.com]
