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. 2020 Jun 7;9(1):1259–1268. doi: 10.1080/22221751.2020.1772678

Table 1. RT-PCR and ddPCR Results of suspected outpatients of COVID-19 and their further clinical information.

Series No. Results of RT-PCR in lab (Ct value)a,b Result of ddPCR (copies/reaction)a,b Judgment by ddPCR Initial official reports by RT-PCRa Further diagnosis by chest CTc Patient
conditiond 		Official reports by RT-PCR again after (days)e
ORF1ab N ORF1ab N
P1 ND ND 0 2.0 P N GGOc Hospitalized P (4d)
P2 39.46 38.90 0 2.0 P N GGO Hospitalized P (4d)
P3 ND ND 0 2.0 P N GGO Hospitalized P (4d)
P4 35.20 35.98 4.8 20.8 P P GGO Hospitalized P (6d)
P5 ND 39.59 0 3.6 P N GGO Hospitalized P (4d)
P6 36.24 35.45 32.4 104 P P GGO Hospitalized P (6d)
P7 ND ND 3.4 13.6 P N GGO Hospitalized P (2d)
P8 36.87 36.10 18.6 36.4 P P GGO Hospitalized P (6d)
P9 39.19 38.87 1.6 13.2 P N GGO Hospitalized P (3d)
P10 ND ND 0 1.6 S N GGO Hospitalized P (4d)
P11 35.08 33.52 4.8 16.4 P P GGO Hospitalized P (6d)
P12 ND ND 0 3.6 P N GGO Hospitalized P (4d)
P13 ND ND 0 4.6 P N GGO Hospitalized P (4d)
P14 38.09 37.63 2.6 3.8 P N GGO Hospitalized P (2d)
P15 ND ND 0 3.6 P N GGO Hospitalized P (2d)
P16 ND ND 0 1.8 P N GGO Hospitalized P (4d)
P17 33.97 32.76 24.4 60.8 P P GGO Hospitalized P (6d)
P18 ND ND 0 7.4 P N GGO Hospitalized P (2d)
P19 ND ND 0 1.8 P N GGO Hospitalized P (4d)
P20 37.77 36.63 16.2 36.2 P P GGO Hospitalized P (6d)
P21 ND ND 3.8 1.8 P N GGO Hospitalized P (3d)
P22 ND ND 2.4 0 P N GGO Hospitalized P (4d)
P23 32.19 31.81 108 132 P P GGO Hospitalized P (6d)
P24 28.02 27.49 1130 4440 P P GGO Hospitalized P (6d)
P25 ND ND 0 1.8 P N GGO Hospitalized P (4d)
P26 ND ND 0 1.4 S N GGO Hospitalized P (5d)
P27 25.33 24.25 544 1384 P P GGO Hospitalized P (6d)
P28 27.02 26.30 22.8 98.2 P P GGO Hospitalized P (6d)
P29 ND ND 0 2.2 P N GGO Hospitalized P (10d)
P30 36.15 36.02 26.2 68.4 P P GGO Hospitalized P (6d)
P31 ND ND 0 6.6 P N GGO Hospitalized P (8d)
P32 34.93 33.52 32.6 64.4 P P GGO Hospitalized P (6d)
P33 ND ND 4.4 14.2 P N GGO Hospitalized P (7d)
P34 28.98 28.69 746 1954 P P GGO Hospitalized P (6d)
P35 ND 39.46 0 3.2 P N GGO Hospitalized P (4d)
P36 31.49 30.63 36.2 140 P P GGO Hospitalized P (6d)
P37 28.22 27.26 20.8 40.4 P P GGO Hospitalized P (6d)
P38 27.09 26.48 8.6 36.2 P P GGO Hospitalized P (6d)
P39 ND ND 0 1.8 P N GGO Hospitalized P (8d)
P40 ND ND 0 3.2 P N GGO Hospitalized P (8d)
P41 32.73 33.18 16.2 11.4 P P GGO Hospitalized P (6d)
P42 34.16 33.09 22.4 32.8 P P GGO Hospitalized P (6d)
P43 25.02 27.30 1.8 2.4 P P GGO Hospitalized P (6d)
P44 37.01 36.57 16.6 28.4 P P GGO Hospitalized P (6d)
P45 35.85 35.26 10.2 32.2 P P GGO Hospitalized P (6d)
P46 ND ND 0 0 N N GGO Hospitalized P (6d)
P47 ND ND 0 0 N N GGO Hospitalized P (8d)
P48 ND 39.27 0 3.4 P N Pleural bleb Home Quarantine NA, lose contact
P49 ND ND 0 1.2 S N Lower-lobe pneumonia Home Quarantine NA, lose contact
P50 ND ND 1.6 4.0 P N Pneumonia Home Quarantine P (10d)
P51 ND 38.83 0 3.8 P N Secondary pulmonary tuberculosis Home Quarantine P (9d)
P52 39.83 38.51 0 5.6 P N Normal Home Quarantine NA, lose contact
P53 39.60 38.16 3.0 16.2 P N Fibrous stripes Home Quarantine P (7d)
P54 ND ND 0 2.0 P N Subpleural nodules Home Quarantine P (11d)
P55 ND ND 0 0 N N Normal Home Quarantine Negative (4, 6 and 8d), asymptomatic
P56 ND ND 0 1.2 S N Normal Excluded N (6d)
P57 ND ND 0 0 N N Emphysema Excluded NA, lose contact
P58 ND ND 0 0 N N Fibrous stripes Excluded N (6d)
P59 ND ND 0 0 N N Normal Excluded N (5d)
P60 ND ND 0 0 N N Normal Excluded NA, lose contact
P61 ND ND 0 0 N N Pneumonia Excluded N (6d)
P62 ND ND 0 0 N N Fibrous stripes Excluded N (6d)
P63 ND ND 0 0 N N Nodules Excluded N (8d)

Note: P, positive; N, Negative; S, suspect; ND, not detected; NA, not applicable.

aThroat swab samples were firstly collected for laboratory RT-PCR, ddPCR tests and official approved RT-PCR diagnosis in hospitals simultaneously using the same primers/probe sets approved by Chinese CDC. In laboratory tests, the RNA of throat swab samples were extracted and reverse transcribed to cDNA that is subjected to both RT-PCR and ddPCR tests subsequently.

bThe reaction systems of laboratory RT-PCR and ddPCR are 20 μl/reaction.

cThe subsequent results of chest CT were used to diagnose the COVID-19 in hospitals based on the official medical programme of China. GGO, ground glass opacities image.

dOutpatients with chest CT (GGO) or official approved RT-PCR (positive) were hospitalized. Outpatients with chest CT (no GGO), official approved RT-PCR (negative) but ddPCR (positive) were suggested to be quarantined at home. Outpatients with CT (normal), official approved RT-PCR (negative), ddPCR (negative) and other supportive clinical tests (supplementary Table S1) were excluded for COVID-19.

eThe infection of SARS-CoV-2 was confirmed by official approved RT-PCR test after the indicated days.