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. 2020 May 29;9(1):1080–1091. doi: 10.1080/22221751.2020.1760735

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Generation of multimeric protein scaffold particles (MPSP)- based vaccines used in this study. (A) Schematic diagram of the MERS-CoV spike (S) protein mapping regions selected for vaccine generation; the receptor binding domain (RBD), the fusion peptide (FP) and heptad repeat 2 (HR2). (B, C) Schematic diagram illustrating the construct design and production of the lumazine synthase (LS) and I3-01 (I3)-based self-assembling MPSP vaccines. (D) Reducing SDS-PAGE showing generation of RBD-LS by covalent coupling of RBD-SpyTag (RBD-ST) and LS-SpyCatcher (LS-SC) at different molar ratios of LS-SC: RBD-ST with the last two lanes showing each in its free (uncoupled) form. (E) Reducing SDS-PAGE analysis of immunogens used in this study. The size of each protein (KDa) is given in Supplementary Table S1. * Fuzzy bands due to heterogeneous glycosylation of HR2 or RBD.