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. 2020 May 29;9(1):1080–1091. doi: 10.1080/22221751.2020.1760735

Figure 4.

Figure 4.

Anti-scaffold antibody responses in sera of vaccinated rabbits. Anti-lumazine synthase (LS) scaffold antibody titres following (A) homologous prime boost in monomeric RBD + LS and multimeric RBD-LS vs heterologous LS/I3 prime boost in control LS/I3 as well as (B) HR2-LS/I3 and FP-LS/I3. Shown are (average ± s.e.m. of n = 5 rabbit/group) antibody titres 4 weeks after prime (day 28, D28) and 3 weeks after boost (day 46, D46) as measured by ELISA. (C) Fold increase (from prime, day 28) in anti-LS antibody titres following boost vaccination (day 46). A paired t-test was performed to determine significant increases in antibody titres post-prime and post-boost within groups (A, B), and an unpaired t-test was performed to determine significant changes in titres between groups (C), with asterisks indicating the level of significance.*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. The dotted lines represent the limits of detection. HR2, heptad repeat 2; FP, fusion peptide; LS, lumazine synthase 60-meric particles; I3, I3-01 60-meric particles; RBD, receptor binding domain; RBD + LS, monomeric uncoupled RBD; RBD-LS, multimeric RBD coupled to LS through covalent SpyTag/SpyCatcher.