Fig. 1.

TW-37 augments the cytotoxicity of human oral cancer cell lines by triggering apoptosis. a The chemical structure of TW-37. b HSC-3 cell lines were treated with 2.5- and 5-μM TW-37 for 48 h, following which the cell viability was measured using the trypan blue exclusion assay. c Cleavage of both PARP and caspase-3 was detected by Western blotting. Actin was used as a loading control. d HSC-3 cell lines were stained with DAPI and visualized by fluorescence microscopy (magnification, ×400). e FACS analysis of annexin V/PI staining. All bar graphs represent mean ± SD of the three independent experiments. *P < 0.05