Table 4.
Summary of CXCR4 panel multiplex immunohistochemistry analysis
| E-Cadherin Panel | Normal (N = 7) | IPF (N = 7) | Mann-Whitney U | Effect Size | ||
|---|---|---|---|---|---|---|
| Mean1 | SD | Mean2 | SD | p-value | ||
| Percentage “cells” = Hoechst+ | 30.69 | 13.6 | 28.91 | 11.5 | 0.209 | |
| Single CXCR4+ | 0.23 | 0.2 | 0.80 | 0.5 | 0.007** | 1.494 |
| Single E-Cadherin+ | 10.59 | 10.5 | 5.20 | 3.9 | 0.318 | |
| Single CXCL12+ | 9.64 | 5.9 | 7.32 | 5.8 | 0.456 | |
| Double CXCR4 + E-Cadherin+ | 0.08 | 0.1 | 0.38 | 0.2 | 0.007** | 1.853 |
| Double CXCR4 + CXCL12+ | 0.15 | 0.2 | 0.38 | 0.1 | 0.049* | 1.349 |
| Double E-Cadherin+CXCL12+ | 4.67 | 3.5 | 3.24 | 2.4 | 0.620 | |
| Triple CXCR4+ E-Cadherin+ CXCL12+ | 0.07 | 0.1 | 0.31 | 0.2 | 0.011* | 1.815 |
| Triple CXCR4+ E-Cadherin- CXCL12- | 0.07 | 0.1 | 0.34 | 0.1 | 0.097 | |
| Triple CXCR4+ E-Cadherin+ CXCL12- | 0.01 | 0.0 | 0.07 | 0.1 | 0.073 | |
| Triple CXCR4+ E-Cadherin- CXCL12+ | 0.09 | 0.2 | 0.07 | 0.2 | 0.383 | |
| Triple CXCR4- E-Cadherin- CXCL12- | 15.06 | 8.3 | 19.30 | 8.9 | 0.535 | |
| Triple CXCR4- ECadherin- CXCL12+ | 4.88 | 4.9 | 4.00 | 5.1 | 0.535 | |
| Triple CXCR4- E-Cadherin+ CXCL12+ | 4.61 | 3.4 | 2.93 | 3.4 | 0.535 | |
| Triple CXCR4- E-Cadherin+ CXCL12- | 5.90 | 7.8 | 1.88 | 7.4 | 0.456 | |
| CD45 Panel | Normal (N = 7) | IPF (N = 7) | Mann-Whitney U | Effect Size | ||
| Mean1 | SD | Mean2 | SD | p-value | ||
| Percentage “cells” = Hoechst+ | 28.13 | 14.6 | 24.57 | 10.9 | 0.456 | |
| Single CXCR4+ | 0.62 | 0.8 | 3.88 | 3.2 | 0.026* | 1.378 |
| Single CD45+ | 18.83 | 9.2 | 12.62 | 9.4 | 0.209 | |
| Single CXCL12+ | 6.40 | 3.8 | 6.15 | 5.1 | 0.710 | |
| Double CXCR4 + CD45+ | 0.17 | 0.1 | 0.78 | 0.8 | 0.026* | 1.041 |
| Double CXCR4 + CXCL12+ | 0.22 | 0.3 | 1.61 | 1.6 | 0.011* | 1.186 |
| Double CD45 + CXCL12+ | 5.28 | 3.0 | 3.49 | 3.9 | 0.209 | |
| Triple CXCR4 + CD45 + CXCL12+ | 0.06 | 0.1 | 0.30 | 0.3 | 0.128 | |
| Triple CXCR4+ CD45- CXCL12- | 0.30 | 0.5 | 1.78 | 1.8 | 0.128 | |
| Triple CXCR4+ CD45+ CXCL12- | 0.11 | 0.1 | 0.48 | 0.2 | 0.097 | |
| Triple CXCR4+ CD45- CXCL12+ | 0.16 | 0.2 | 1.32 | 0.7 | 0.011* | 2.245 |
| Triple CXCR4- CD45- CXCL12- | 7.88 | 6.4 | 7.52 | 4.6 | 0.902 | |
| Triple CXCR4- CD45- CXCL12+ | 0.96 | 0.9 | 1.34 | 0.9 | > 0.9999 | |
| Triple CXCR4- CD45+ CXCL12+ | 5.22 | 2.9 | 3.20 | 3.4 | 0.209 | |
| Triple CXCR4- CD45+ CXCL12- | 13.45 | 9.2 | 8.64 | 8.1 | 0.318 | |
Multiplex immunohistochemistry was performed, and cell phenotypes were quantified. The percentage of each cell phenotype in normal (n = 7) and idiopathic pulmonary fibrosis (IPF, n = 7) lung tissue was compared using Mann-Whitney U test. Effect size (Cohen’s D) was calculated for comparisons that were significantly different (*p < 0.05, **p < 0.01). Avg, average