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. 2020 Aug 26;21:591. doi: 10.1186/s12864-020-07004-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Schematic of the two RT-qPCR assays for each junction in DGAT1. The blue boxes represent exons while the blue line represents the intron. The green line represents the probe, while the orange and purple arrows represent the primers for unspliced and spliced mRNA transcripts, respectively. The first assay quantifies the intron containing pre-mRNA transcripts (orange) while the second assay quantifies the spliced mRNA transcripts (purple). The ratio of mRNA:pre-mRNA transcripts is used to generate a splicing efficiency phenotype for each junction