KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal anti-HA clone 3F10 | Roche | Cat# 11867432001; RRID: AB_2314622 |
| Rabbit monoclonal anti-MeCP2 | Cell Signaling | Cat# 34456; RRID: AB_2143849 |
| Mouse monoclonal Anti-HA | Biolegend | Cat# 901514; RRID: AB_2565336 |
| Rabbit polyclonal Anti-Histone H3 | Abcam | Cat# Ab1791; RRID: AB_302613 |
| Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21208; RRID: AB_2535794 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-31573; RRID: AB_2536183 |
| Goat anti-mouse IgG DyLight IR 680 | Thermo Fisher Scientific | Cat# 35518; RRID: AB_614942 |
| Goat anti-rabbit IgG Dylight IR 800 | Thermo Fisher Scientific | Cat#SA5–10036; RRID:AB_2556616 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| TRIzol | Thermo Fisher Scientific | Cat# 15596026 |
| Poly(L) lysine hydrobromide | Sigma Aldrich | Cat# P2636 |
| 2,2,2-tribromoethanol | Sigma Aldrich | Cat# T48402 |
| 4’,6’-diamidino-2-phenylindole (DAPI) | Thermo Fisher Scientific | Cat#D1306 |
| Critical Commercial Assays | ||
| SMARTer RNA kit | Clonetech | Cat# 634940 |
| Seq-Cap Exome Plus capture kit | Roche | Cat# 06740189001 |
| KAPA Library Quantification kit | Roche | Cat# 7960140001 |
| NucleoBond Xtra Maxi Endotoxin Free Kit | Takara Bio | Cat #740424.10 |
| RNeasy Micro kit | QIAGEN | Cat# 74004 |
| Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| Invitrogen Superscript III First-Strand Synthesis System | Thermo Fisher Scientific | Cat# 18080051 |
| QIAquick gel extraction kit | QIAGEN | Cat# 28706 |
| Agilent RNA 6000 Pico Kit | Agilent | Cat#5067–1513 |
| PureLink Genomic DNA Isolation kit | Thermo Fisher Scientific | Cat#K182001 |
| Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | Cat# 11668019 |
| Deposited Data | ||
| Whole transcriptome RNA-seq | This paper | SUB7012760 |
| Whole exome sequencing | This paper | SUB7012760 |
| Experimental Models: Cell Lines | ||
| HEK293 Cells (AAV-293) | Agilent | cat# 240073; RRID: CVCL_6871) |
| Neuro-2A Cells (N2A) | ATCC | Cat#CCL-131; RRID: CVLCL-0470 |
| Experimental Models: Organisms/Strains | ||
| Mecp2317G > A; C57BL/6J mice | Sinnamon et al., 2017 | NA |
| C57BL/6J | The Jackson Laboratory | JAX Stock 000664 |
| Oligonucleotides | ||
| Guide sequences; see Table S1 | This paper | NA |
| PCR and sequencing primers; see Table S1 | This paper | NA |
| Primers for cloning of pGM1267; see Table S1 | This paper | NA |
| Recombinant DNA | ||
| Plasmid: P5E18-VD2/9 | Gao et al., 2002 | NA |
| Plasmid: PHP.B 7-mer peptide coding DNA sequence | Deverman et al., 2016 | NA |
| Plasmid: pGM1258 | Sinnamon et al., 2017 | NA |
| Plasmid: pGM1186 | Sinnamon et al., 2017 | NA |
| Plasmid: pGM1108 | Sinnamon et al., 2017 | NA |
| Plasmid: pGM1267 | This paper | NA |
| Software and Algorithms | ||
| ImageJ, Version 1.60_65 (32bit) | https://imagej.nih.gov/ij/ | RRID: SCR_003070 |
| Bioedit Software Package | https://bioedit.software.informer.com/7.2/ | RRID: SCR_007361 |
| Zen Digital Imaging Software | Zeiss | RRID: SCR_013672 |
| Bwa-mem 0.717 | http://bio-bwa.sourceforge.net | RRID: SCR_010910 |
| Bowtie 1.2.2. | https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.2.2 | RRID: SCR_005476 |
| REDItoolDNARNA | Picardi and Pesole 2013 | RRID: SCR_012133 |
| Graph Pad Prism 6.0e | Graph Pad | RRID: SCR_002798 |
| Other | ||
| Tissue Freezing Medium | Electron Microscopy Sciences | Cat# 72592 |
| Pen 1.0 membrane slides | Zeiss | Cat# 415190-9041-000 |
| ProLong Gold Antifade Mountant | Thermo Fisher Scientific | Cat# P36934 |
| Normal Donkey Serum | Jackson Immunoresearch Labs | Cat# 017-00-001 |