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. 2020 Aug 20;64(9):e00535-20. doi: 10.1128/AAC.00535-20

FIG 5.

FIG 5

Efficacy of oral treatment with ELQ-334 against established T. gondii brain cysts. (A) Mice were infected for 5 weeks with MS-ME49 prior to daily treatment for 2 weeks. (B) Number of T. gondii brain cysts in mice treated with 3 mg/kg and 10 mg/kg of ELQ-334. The indicated P values are from an ordinary analysis of variance with multiple comparisons and Tukey’s correction. One outlier was removed from the vehicle-treated group based on ROUT analysis (Q value, 0.1%). The cyst values in the groups treated with the vehicle, ELQ-334 at 3 mg/kg, and ELQ-334 at 10 mg/kg are from 12, 15, and 16 mice, respectively. (C) Number of genomes per brain in mice treated with ELQ-334. The indicated P values are from an ordinary analysis of variance with multiple comparisons and Tukey’s correction. One outlier was removed from the vehicle-treated group based on ROUT analysis (Q value, 0.1%). Data are from 7 vehicle-treated mice and 8 ELQ-334-treated mice. (D) Diameter of cysts from mice treated with ELQ-334. The P value is from a Mann-Whitney test. Data are from 5 brain samples per group, with 13 to 21 cysts being measured per sample. (E) Distribution of T. gondii in the brains of infected mice. Data are from 8 mice per group for the vehicle- and ELQ-316-treated groups. One mouse was used for the uninfected control group. (F) Regraphing of the distribution of T. gondii in the brains of infected mice treated with ELQ-334 to visualize the pattern and its similarity to that for mice treated with the vehicle, as shown in panel E. (G) Mice were infected with cysts from the ELQ-334-treated mice for 5 weeks prior to retreatment with ELQ-334 for 2 weeks. (H) ELQ-334 treatment of mice infected with cysts from mice that had previously been treated with ELQ-334. P values are from a Mann-Whitney test. Cyst levels in the vehicle-/vehicle-treated (Veh/Veh), vehicle-/ELQ-334-treated (Veh/ELQ), ELQ-334-/vehicle-treated (ELQ/Veh), and ELQ-334-/ELQ-334-treated (ELQ/ELQ) groups are from 9, 6, 6, and 9 mice, respectively. (I) Comparison of the cytochrome b Qo site sequence of the MS-ME49 strain to that of the CH-ME49 strain. (J) Mice infected with T. gondii CH-ME49 cysts were treated with ELQ-334 (n = 13) or vehicle only (n = 8). The P value is from a Mann-Whitney test. PO, per os; error bars, standard deviations.