TABLE 2.
Resistance index for the AP-selected lines after the highest exposurea
| Compound | Extracellular promastigote assay |
Intracellular amastigote assay |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild type vs LEM3323Cl4/1044 |
Wild type vs LEM3323Cl4/8012 |
Wild type vs LEM3323Cl4/1044 |
Wild type vs LEM3323Cl4/8012 |
|||||||||
| Mean IC50 (μM) ± SEM |
RI | Mean IC50 (μM) ± SEM |
RI | Mean IC50 (μM) ± SEM |
RI | Mean IC50 (μM) ± SEM |
RI | |||||
| Wild type | LEM3323Cl4/1044 | Wild type | LEM3323Cl4/8012 | Wild type | LEM3323Cl4/1044 | Wild type | LEM3323Cl4/8012 | |||||
| DNDI-1044 | 0.20 ± 0.04 | 4.90 ± 0.55 | 24.5 | 0.34 ± 0.11 | 2.78 ± 0.55 | 8.2 | 0.44 ± 0.12 | 2.01 ± 0.46 | 4.6 | 0.29 ± 0.01 | 0.37 ± 0.01 | 1.3 |
| DNDI-8012 | 0.52 ± 0.02 | 7.55 ± 1.30 | 14.5 | 0.62 ± 0.12 | 3.62 ± 0.31 | 5.8 | 0.54 ± 0.10 | 2.20 ± 0.35 | 4.1 | 0.22 ± 0.00 | 0.19 ± 0.01 | 0.9 |
a
The results are based on two independent replicates run in duplicate. The intracellular assay with LEM3323Cl4/8012 was performed only once in duplicate, as no differences were observed. RI, resistance index.