TABLE 6.
Overview of common mutated genes in both LEM332Cl4/1044Cl1 and LEM3323Cl4/8012Cl2a
| LEM3323Cl4 strain | Gene identifier | Position of mutation | Ref/mut nucleotide | Allele frequency | Ref/mut AA | Gene function |
|---|---|---|---|---|---|---|
| 1044Cl1 | LinJ.03.0410 | 156 | GA/G | Hom. | NA | 60S acidic ribosomal protein P2, putative |
| 8012Cl2 | 156 | GA/G | Hom. | NA | ||
| 1044Cl1 | LinJ.10.0390 | 172 | G/A | Hom. | E/K | Folate biopterin transporter, putative |
| 8012Cl2 | 172 | G/A | Hom. | E/K | ||
| 1044Cl1 | LinJ.12.0070 | 1277 | G/C | Het. | R/P | Hypothetical protein, unknown function |
| 8012Cl2 | 1277 | G/C | Het. | R/P | ||
| 1044Cl1 | LinJ.14.1180 | 2511 | G/C | Het. | E/D | Kinesin K39, putative |
| 8012Cl2 | 1885 | T/G | Het. | S/A | ||
| 8012Cl2 | 2511 | G/C | Het. | E/D | ||
| 1044Cl1 | LinJ.14.1190 | 3770 | A/C | Het. | E/A | Kinesin K39, putative |
| 8012Cl2 | 3770 | A/C | Het. | E/A | ||
| 8012Cl2 | 5699 | G/A | Hom. | S/N | ||
| 1044Cl1 | LinJ.15.0290 | 2429 | T/G | Het. | L/R | Hypothetical protein, conserved |
| 8012Cl2 | 561 | C/CGTCTCGGAAGCGGAGTCGCTCTCAGCCGCG | Het. | NA | ||
| 1044Cl1 | LinJ.18.1290 | 283 | T/G | Het. | S/A | Hypothetical protein, conserved |
| 8012Cl2 | 283 | T/G | Het. | S/A | ||
| 1044Cl1 | LinJ.19.1690 | 957 | AGCGCCCCAGCCGAGCGAGGCGGCGCCGGTGTCTGCAGTGGAGGCTCTGCCTCCGACGCCTGCCGAGTGCGCATCTGAGGCG/A | Het. | NA | Hypothetical protein |
| 8012Cl2 | 957 | AGCGCCCCAGCCGAGCGAGGCGGCGCCGGTGTCTGCAGTGGAGGCTCTGCCTCCGACGCCTGCCGAGTGCGCATCTGAGGCG/A | Het. | NA | ||
| 1044Cl1 | LinJ.27.2060 | 294 | T/TG | Het. | NA | Hypothetical protein, unknown function |
| 8012Cl2 | 294 | T/TG | Het. | NA | ||
| 1044Cl1 | LinJ.29.0270 | 546 | T/G | Het. | E/D | Hypothetical protein, conserved |
| 8012Cl2 | 546 | T/G | Het. | E/D | ||
| 8012Cl2 | 544 | C/G | Het. | E/Q | ||
| 1044Cl1 | LinJ.29.2240 | 1267 | G/A | Hom. | L/F | Hypothetical protein, conserved |
| 8012Cl2 | 1267 | G/A | Hom. | L/F | ||
| 1044Cl1 | LinJ.31.1290 | 3139 | G/C | Het. | R/G | P-glycoprotein e ABCC4 |
| 8012Cl2 | 3350 | A/C | Het. | L/R | ||
| 8012Cl2 | 3139 | G/C | Het. | R/G | ||
| 1044Cl1 | LinJ.32.1310 | 827 | G/C | Het. | S/T | Ubiquitin hydrolase, putative; cysteine peptidase Clan CA family C19, putative |
| 8012Cl2 | 827 | G/C | Het. | S/T | ||
| 1044Cl1 | LinJ.33.3230 | 5873 | A/G | Het. | Y/C | Hypothetical protein, conserved |
| 8012Cl2 | 5873 | A/G | Het. | Y/C | ||
| 1044Cl1 | LinJ.34.0710 | 2417 | T/C | Het. | K/R | Flagellar attachment zone protein, putative |
| 8012Cl2 | 2417 | T/C | Het. | K/R | ||
| 1044Cl1 | LinJ.34.2650 | 544 | T/G | Het. | S/A | Amastin-like surface protein, putative |
| 8012Cl2 | 544 | T/G | Het. | S/A | ||
| 1044Cl1 | LinJ.35.0490 | 11527 | G/A | Het. | A/T | Proteophosphoglycan ppg4 |
| 8012Cl2 | 11527 | G/A | Het. | A/T | ||
| 1044Cl1 | LinJ.35.0510 | 721 | G/A | Hom. | A/T | Proteophosphoglycan ppg4 |
| 8012Cl2 | 721 | G/A | Het. | A/T | ||
| 8012Cl2 | 733 | C/G | Het. | L/V | ||
| 1044Cl1 | LinJ.35.0520 | 6859 | G/A | Het. | G/S | Proteophosphoglycan ppg4 |
| 8012Cl2 | 6859 | G/A | Het. | G/S | ||
| 1044Cl1 | LinJ.35.0540 | 685 | T/C | Het. | C/R | Proteophosphoglycan 5 |
| 8012Cl2 | 685 | T/C | Het. | C/R |
a
Ref/mut, reference/mutated; Hom., homozygous; Het., heterozygous; NA, not applicable due to frameshift; AA, amino acid.