. 2020 Aug 20;64(9):e00890-20. doi: 10.1128/AAC.00890-20

TABLE 2.

Daptomycin susceptibility and mprF polymorphisms with β-lactam passage after 28 days^a

Isolate	Passage	Replicate	DAP MIC (mg/liter)	β-lactam MIC (mg/liter)^b	mprF SNP	MprF domain	div1b mutation	rpoB/C mutation
J01	None		0.5
J03	None		2		T₃₄₅I	Bifunctional		rpoB S₄₆₄P
	Media	i	2		None
	Media^a	ii	1		Y₃₂₅H	Bifunctional
	Media^a	iii	1		R₄₃₇P	Synthase
	CRO^c	ii	0.75	512	V₁₅₂G	Translocase
	LOX^c	ii	0.125	32	R₇₈₈L	Synthase	Q₄₂₅^d
	LOX^c	iii	0.125	32	R₇₈₈L	Synthase	Q₄₁₅^d
D592	None		0.5
D712	None		2		L₃₄₁S	Bifunctional
	Media	i	2		None
	Media	ii	1		None
	Media	iii	2		None
	NAF^e	iii	1	256	S₃₃₇P	Bifunctional
	CRO^e	i	2	2,048	M₆₀₉T	Synthase		rpoC A₅₆₇V
	CRO^e	iii	0.5	2,048	G₃₈₉A	Synthase		rpoB G₇₆₇C
	FOX^e	ii	0.5	512	F₆₅₇L	Synthase
	LOX^e	i	0.5	1,024	S₁₃₆L	Translocase
	LOX^e	ii	0.5	1,024	S₁₃₆L	Translocase
C24	None		0.5		-	-
C25	None		3–4		S₂₉₅L	Bifunctional
	Media^f	i	1		S₈₂₅^d	Synthase
	Media^f	ii	1		S₈₂₅^d	Synthase
	Media	iii	2		None	-
	FOX^f	Iii	1	64	A₃₁₅S	Bifunctional
	LOX^f	i	0.125	8	L₈₄^d	Translocase	A₄₂₀E
	LOX^f	ii	0.125	8	L₈₄^d	Translocase
	LOX^f	iii	0.125	8	L₈₄^d	Translocase	E₄₁₆^d

For the β-lactam-exposed strains, only the passages with additional mprF mutations (versus DAP-NS parent strain) are presented.

β-lactam MIC represents the MIC in the respective β-lactam after passage.

Passage isolates maintained MprF T₃₄₅I and RpoB S₄₆₄P.

Nonsense stop-gain mutation resulting in premature end of translation.

Passage isolates maintained MprF L₃₄₁S.

Passage isolates maintained MprF S₂₉₅L.