TABLE 1.

Carbapenemase gene and plasmid curing

Strain	ST	Carbapenemase gene and/or plasmid	Reference or source	Target gene(s)	Target sequence(s) (N20)	Curing efficiency (%)b
Strains containing plasmid and resistance genes
K. pneumoniae 13001	258	blaKPC-2, IncFIIK-pKpQIL	25	IncFIIK repA	GGTTTTTAACCTGTGAATAG	98.6 ± 2.4
				IncFIB(pQIL) repB	ACTGACTTCAAGGTGTGGGT	100 ± 0
				parA	TAAGGTATTTCAACGTGGAA	99.3 ± 1.2
				blaKPC	CAATTTGTTGCTGAAGGAGT	98.6 ± 1.2
K. pneumoniae Kp97_58	111	blaKPC-2, IncN	10	IncN repA	GAGCAAGACTTTCATAAAGG	97.9 ± 2.1
				blaKPC	CAATTTGTTGCTGAAGGAGT	96.5 ± 2.4
E. coli 53433	167	blaNDM-5, IncX3	This study	IncX3 repA	AATGTTAGATGATGATATTT	99.3 ± 1.2
				blaNDM	CCCAACGGTGATATTGTCAC	97.2 ± 4.8
K. pneumoniae 49210	23	blaOXA-48, IncL- pOXA48	This study	IncL repA	ATATCATGCGTTTCTAAGCG	100 ± 0
				blaOXA-48-like	CACCAAGTCTTTAAGTGGGA	99.3 ± 1.2
				IncL repA and blaOXA-48-likec	ATATCATGCGTTTCTAAGCG and CACCAAGTCTTTAAGTGGGA	100 ± 0c
Strains containing resistance genes
E. hormaechei 34978	171	blaKPC-3	26	blaKPC	CAATTTGTTGCTGAAGGAGT	95.8 ± 2.1
E. xiangfangensis 34399	114	blaKPC-3	26	blaKPC	CAATTTGTTGCTGAAGGAGT	95.1 ± 2.4
E. coli 28009	131	blaKPC-2	27	blaKPC	CAATTTGTTGCTGAAGGAGT	97.2 ± 3.2
S. marcescens SmN01	a	blaNDM-7	28	blaNDM	CCCAACGGTGATATTGTCAC	94.4 ± 3.2
K. pneumoniae 51933	307	blaOXA-48	29	blaOXA-48-like	CACCAAGTCTTTAAGTGGGA	98.6 ± 1.2

^aNot examined.

^bThe values shown are the mean ± SD of three independent experiments.

^cThe double sgRNAs were generated by inserting the IncL sgRNA in pCasCure-OXA48-Apr at the PstI and XmaI restriction sites. The curing result was obtained at 4 h of incubation with 0.1% arabinose treatment.