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. 2020 Aug 20;64(9):e00852-20. doi: 10.1128/AAC.00852-20

FIG 3.

FIG 3

S1-PFGE and Southern hybridization analysis of aac(3)-IIg-bearing conjugative and nonconjugative plasmids. (A, B) S1-PFGE analysis of plasmids from 23 transconjugants (A) and Southern hybridization with an aac(3)-IIg-specific probe (B). (C) S1-PFGE (left) and Southern blotting (right) of plasmid DNA of isolate Y233 and its transconjugant, JY233. (D, E) S1-PFGE patterns of 13 E. cloacae complex isolates that could not transfer aminoglycoside resistance to recipients by conjugation (D) and Southern hybridization with an aac(3)-IIg-specific probe (E). Lanes M, molecular size markers.