TABLE 2.
MICs of various aminoglycosides for individual recombinant E. coli DH5α isolates producing AAC(3)-IIg and the corresponding 3 aac(3)-IIg-positive isolates
| Strain | MIC (μg/ml)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GEN | KAN | TOB | SIS | MCR | NET | STR | SPE | AMK | NEO | RIB | APR | |
| DH5α | ≤0.25 | 1 | 0.5 | ≤0.5 | ≤0.5 | 0.5 | 2 | 4 | ≤1 | 1 | 2 | 4 |
| DH5α/pMD19T-aac(3)-IIg (Y108) | 512 | 8 | 32 | 512 | 1,024 | 2 | 2 | 8 | ≤1 | 1 | 2 | 4 |
| DH5α/pMD19T-aac(3)-IIg (Y315) | 512 | 8 | 64 | 512 | 1,024 | 4 | 2 | 8 | ≤1 | 1 | 2 | 4 |
| DH5α/pMD19T-aac(3)-IIg (Y2152) | 512 | 8 | 64 | 512 | 1,024 | 4 | 2 | 4 | ≤1 | 1 | 2 | 4 |
| Y108 | 128 | 64 | 64 | 256 | 512 | 128 | 128 | 16 | 8 | 2 | 512 | 4 |
| Y315 | 256 | 256 | 128 | 1,024 | 1,024 | 64 | 128 | 16 | 8 | 16 | 1,024 | 4 |
| Y2152 | 64 | 128 | 4 | 128 | 256 | 1 | 128 | 4 | ≤1 | 16 | 512 | 4 |
a
Abbreviations: GEN, gentamicin; KAN, kanamycin; TOB, tobramycin; SIS, sisomicin; MCR, micronomicin; NET, netilmicin; STR, streptomycin; SPE, spectinomycin; AMK, amikacin; NEO, neomycin; RIB, ribostamycin; APR, apramycin.