Figure 1.

Laboratory ²H-enrichment Box plot. Non-enriched control adult mosquitoes (blue), 10-day-old enriched (red), and 1 day-old enriched (green). Experiment 1; Direct supplementation of ²H-deuterium-oxide into mosquito larvae rearing water. Only values of longest exposure to deuterated conditions (5 days) shown, 1-day-old adults are presented (Controls; n=6, 10-day old; n=6 and 1-day old; n=16). Experiment 2; Deuterated microbiota culture fed to larvae reared in 0.1 v/v% deuterated rearing water. Results from 1-day-old adults (Controls; n=4 and 1-day old; n=13). Experiment 3; Deuterated microbiota culture fed to larvae in 0.1 v/v% deuterated water with additional ²H-spiking at days 3 and 6 post hatch (Controls; n=6, 10-day old; n=3 and 1-day old; n=6). Enrichment threshold (dashed red line, 140 ppm) was set at the level of the highest control value (rounded up to next integer). Diamonds inside boxes denote the means, horizontal line denote medians (corresponding value shown). Whiskers are 25 (bottom) and 75 (top) percentiles.