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. Author manuscript; available in PMC: 2020 Aug 26.
Published in final edited form as: Methods Ecol Evol. 2019 May 17;10(8):1274–1285. doi: 10.1111/2041-210x.13210

Figure 3.

Figure 3.

Estimated 2H signal attrition functions with mosquito age (computed using Proc GLIMMIX, see Materials and Methods). Note that the variance among individual mosquitoes (circles) also reflects their cohort, larval-site, and dose. Extrapolation (dashed colored curves) of the effect of age on 2H values up to 240 days (~8 months) post-emergence from enriched sites based on experimental data from field-collected mosquitoes at the range of 1–50 days (see inset). According to this extrapolation, initial 2H enrichment levels between 240–255 ppm in A. gambiae s.l. may potentially enable detection of 50% of enriched individuals up to 180 days (6 months) after eclosion. Dashed horizontal red line denotes the threshold (144 ppm) we used to separate the natural 2H abundance and the experimentally enriched.