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. 2020 Aug 26;26:e20200016. doi: 10.1590/1678-9199-JVATITD-2020-0016

Figure 10. Immunorecognition profile obtained of individual C. d. collilineatus and C. d. terrificus venom by anticrotalic antivenom produced by Butantan Institute by Western blotting. Venom proteins (20 μg) were subjected to SDS-PAGE 15% under reducing conditions and electrotransferred to a PVDF membrane. Membrane was sequentially incubated with anti-bothropic antivenom and peroxidase-conjugated anti-horse IgG. The reaction was developed using DAB and H2O2. MW: molecular weight marker (Dual Color Precision Plus Protein Standards - BioRad); C. d. collilineatus GO: specimens from Goiás state; C. d. collilineatus SP: specimens from São Paulo state; C. d. terrificus MG: specimens from Minas Gerais state; C. d. terrificus SP: specimens from São Paulo state.

Figure 10.