Extended Data Fig. 4. Bcl-6 drives CXCR5 expression via repression of Id2-E2A pathway.

Related to Fig. 4

a, Quantification of GC-T_FH core signature markers in SMARTA cells from dLNs of KLH-gp₆₁ immunized mice in Fig. 4d.

b, Schematic of the SMARTA cell transfer system used for LCMV_Arm infection. WT, Bcl6^f/fPrdm1^f/f, or Bcl6^f/fPrdm1^f/fId2^f/f Cre^CD4 SMARTA CD4 T were transferred to C57BL/6 host mice, followed by infection of the host mice with LCMV_Arm, and analyzed 7 days later. Related to Fig 4g and Extended Data Fig. 4c–g.

c,e, Representative flow cytometry of T_FH and GC-T_FH SMARTA cell subsets from spleen of LCMV_Arm infected mice. Two independent experiments performed; each dot represents one mouse (n = 4). Data are mean ± s.d., unpaired two-tailed Student’s t-test.

d, Quantification of expression of CXCR5 in Extended Data Fig. 4c, gated on SMARTA cells.

f, Quantification of frequency of CXCR5^hiPD1^hi GC-T_FH cells in Extended Data Fig.4e, gated on SMARTA cells.

g, Quantification of GC-T_FH core signature markers in Extended Data Fig.4e, gated on SMARTA cells.